LAUSR

The present study aimed to transfer nod and nif genes from Rhizobium and Azotobacter to Bacillus by: the transformation technique which used to transfer nodD2 and nodD3 from Rhizobium leguminosarum to Bacillus megaterium; and the conjugation mechanism which performed the transfer of nifH2 and nifH3 from Azotobacter chroococcum to transformant Bacillus megaterium. The results showed that: 12 colonies were obtained after transformation on Sperber’s agar plates which contained tetracycline and ampicillin. While after conjugation; 166 colonies were obtained on Sperber’s agar plates which contained ampicillin and erthymycinem, indicating that the transformation and conjugation were successful. To confirm this, a molecular study was performed based on the followings: the extraction of the plasmid DNA from Rhizobium leguminosarium and transformant Bacillus megaterium to detect nodD genes; and the extraction of the genomic DNA from Azotobacter chroococcum and transformantconjugant Bacillus megaterium to detect nifH genes by PCR and gel electrophoresis. The PCR products on gel electrophoresis showed that Rhizobium leguminosarium and transformant Bacillus megaterium contained nodD2 and nodD3; and Azotobacter chroococcum and transformant-conjugant Bacillus megaterium contained nifH2 and nifH3. Furthermore, the new Bacillus megaterium which obtained these genes successfully, can be used as biofertilizer for nitrogen fixation and phosphorus solubilization at the