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ANNOTATION AND ANALYSIS OF THE TRANSCRIPTOME OF BEARING BRANCH

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The analysis of the Transcriptome of different jujube, and the mining of their functional genes was performed. And the genes were annotated and analyzed, and the difference gene was screened… Click to show full abstract

The analysis of the Transcriptome of different jujube, and the mining of their functional genes was performed. And the genes were annotated and analyzed, and the difference gene was screened out to provide the theoretical basis for the fresh jujube. The sequencing of the cDNA library was performed using synthesis technology and the Illumina HiSeq platform. The sequencing results of splicing and functional annotation. A total of 29402 Unigenes were annotated, while in the Nr, Swiss-Prot, GO, COG, KEGG and Pfam databases 29330, 24358, 21771, 11028, 6962 and 28940 Unigenes, respectively. At the same time, 355 new genes were annotated. The 50 functional groups in the Go database gene function annotation, 14608 metabolic processes in biological processes, accounting for 67.1%; reference KEGG database, 110 metabolic pathways, the number of annotations, gene function ribosomes, amino acid biosynthesis. Human embryonic stem cells, carbon metabolism, and plant hormone signaling are more abundant; it is noted that COD in 11028 single-gene distribution families has general function prediction, transcription, replication, recombination and repair, and secondary metabolites in 25 genes. Biosynthesis, transport and catabolism. This study is the first time to use RNA-seq high-throughput sequencing technology to perform transcriptome sequencing and functional analysis of transcriptome results in fresh-eating jujube leaves, to explore genes for biological processes, cell components and molecular functions, and to study different functional genes, effective biosynthetic pathways and regulatory mechanisms.

Keywords: analysis; annotation analysis; gene; analysis transcriptome

Journal Title: Applied Ecology and Environmental Research
Year Published: 2019

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