LAUSR

In this study, the key factors affecting the formation of humic substances (HS) during green waste composting(GWC) and their potential relationships were investigated by setting up different initial particle sizes (IPS) (2 and 5 mm) and C/N (23, 30, and 37) for composting, evaluating the dynamic of environmental factors and humification parameters during the composting, and performing high-throughput sequencing to detect the bacterial structure and diversity dynamics. The results showed that the 2 mm and C/N=23 treatment could promote the degradation of organic matter, increase the fermentation temperature and germination index (GI), and generate a high HS content and humification degree, indicating that the IPS and C/N ratio were the potential controlling factors for composting maturation. Taxonomic analysis showed that the bacterial community structure was significantly different at various stages of composting, while the bacterial community composition of the same composting stage for the different treatments was similar. In addition, a correlation analysis and redundancy analysis showed that the formation of HS was significantly affected by temperature, GI, polyphenols and polysaccharides. Anaerolineales, Cytophagales and Blastocatellales were the important microbial markers of the maturation process. The above results provide insights and potential methods for regulating HS formation during the composting process.