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INTRODUCTION The microscopic examination of microhematocrit tubes (mHCT) has been proposed as the gold standard for acute and congenital Chagas disease diagnosis. We compared different mHCT methodologies detecting T. cruzi… Click to show full abstract
INTRODUCTION The microscopic examination of microhematocrit tubes (mHCT) has been proposed as the gold standard for acute and congenital Chagas disease diagnosis. We compared different mHCT methodologies detecting T. cruzi parasites in the blood. METHODS The rotating method, water mount, and immersion oil methods were compared for their suitability, sensitivity, and specificity. RESULTS The rotating method was easier, faster, and more sensitive than the others with 100% specificity. CONCLUSIONS The rotating method is feasible for laboratory technicians with standard training in microscopic techniques and is recommended for the diagnosis of acute Chagas disease in primary health care facilities.
Journal Title: Revista da Sociedade Brasileira de Medicina Tropical
Year Published: 2019
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