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Vesicular stomatitis due to Indiana III (Alagoas/VSIV-3) is endemic in Brazilian state of Ceará

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EnglishAlthough Northeast Brazil is considered free of foot and mouth disease (FMD) with vaccination, several economic and health damages are still recorded due to the occurrence of vesicular syndromes that… Click to show full abstract

EnglishAlthough Northeast Brazil is considered free of foot and mouth disease (FMD) with vaccination, several economic and health damages are still recorded due to the occurrence of vesicular syndromes that can be evaluated, such as Vesicular Stomatitis (VS). Therefore, this study aimed to confirm the occurrence of this disease and to determine the predominant viral serotype in suspected cases notified to the Official Veterinary Service of Ceara in 2013 performing official diagnostic protocols recommended by the World Organization for Animal Health. After clinical and epidemiological investigation in 46 farms, 32 probable cases of VS were considered with 78 sampled animals, 65 bovines and 13 equines. Serum (54) and epithelium (24) samples were collected. Six (14.6%) of 41 bovines and 8 (61.5%) of 13 equines described seroconversion to Indiana Vesiculovirus (IVV) by viral neutralization. The IVV was detected in 15 (62.5%) of 24 bovines epithelia using the indirect sandwich ELISA. Finally, positive epithelium underwent complement fixation test viral subtyping that identified the occurrence of Indiana III serotype (Alagoas/IVV-3) in 11 (73.3%) of 15 previous positives cattle. These were the first confirmed cases of VS in Ceara with an official diagnosis of IVV-3, confirming the endemic character attributed to the state through previous unofficial serological surveys. The presence of VS is a continuing diagnostic challenge, given the risk of possible incursions of FMD. Vesicular stomatitis is recurrent and is a worrying in this area free of foot and mouth disease with vaccination that bring damage to producers and a maximum alert to the Sanitary Defense Organs in the face of a probable case of vesicular syndrome portuguesEmbora o Nordeste do Brasil seja considerado livre de Febre Aftosa (FA) com a vacinacao, ainda sao registrados varios prejuizos economicos e sanitarios devido a ocorrencia de sindromes vesiculares que precisam ser adequadamente avaliadas, como Estomatite Vesicular (EV). Portanto, este estudo teve como objetivo confirmar a ocorrencia desta doenca e determinar o sorotipo viral predominante em casos suspeitos notificados ao Servico Veterinario Oficial do Ceara no ano de 2013 realizando protocolos oficiais de diagnostico recomendados pela Organizacao Mundial de Saude Animal. Apos investigacao clinica e epidemiologica em 46 propriedades rurais, foram considerados 32 casos provaveis de propriedade foco de EV com 78 animais amostrados, sendo 65 bovinos e 13 equideos. Amostras de soro (54) e epitelio (24) foram coletadas. Analises sorologicas de 6 (14,6%) de 41 bovinos e 8 (61,5%) de 13 equideos apresentaram soroconversao ao Vesiculovirus Indiana (VVI) por neutralizacao viral. O VVI foi detectado em 15 (62,5%) de 24 epitelios bovinos usando ELISA indireto sanduiche. Por fim, amostras de epitelio positivas foram submetidas a subtipagem viral por fixacao do complemento que identificou a ocorrencia do sorotipo Indiana III (Alagoas/VVI-3) em 11 (73,3%) de bovinos. Estes foram os primeiros casos confirmados de EV no estado do Ceara com diagnostico oficial de VVI-3 confirmando o carater endemico atribuido ao Estado por meio de levantamentos sorologicos nao oficiais anteriores. A presenca de EV e um desafio diagnostico continuo, dado o risco de possiveis incursoes de FA. A Estomatite Vesicular e recorrente e preocupante nesta area livre de FA com vacinacao pois, alem de prejuizos aos produtores, traz um alerta maximo aos Orgaos de Defesa Sanitaria diante de um caso provavel de sindrome vesicular.

Keywords: alagoas; indiana; vesicular stomatitis; indiana iii

Journal Title: Ciencia Rural
Year Published: 2020

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