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Isolation, molecular and phylogenetic identification of microorganisms from Kombucha solution and evaluation of their viability using flow cytometery

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In last decade, nondairy herbal drinks have attracted the attention of consumers and researchers due to their beneficial effects on health resulted from their content of polyphenols (Annuzzi et al.,… Click to show full abstract

In last decade, nondairy herbal drinks have attracted the attention of consumers and researchers due to their beneficial effects on health resulted from their content of polyphenols (Annuzzi et al., 2014). Kombucha is a fermented probiotic and carbonated beverage produced by co-cultures of bacteria and osmophilic yeasts in sweeten tea (Amarasinghe et al., 2018; Yikmis & Tuggum, 2019; Gaggia et al., 2018; Zhao et al., 2018; Kozyrovska et al., 2012). Fermentation of kombucha includes production of alcohol, lactic and acetic acid (VillarrealSoto et al., 2018). in the first days of fermentation, glucose and fructose will be produced from sucrose by the microbial invertase enzyme. Then yeast convert glucose to CO2 and ethanol (Sreeramulu et al., 2000; Loncar et al., 2006). Acetobacteria play their role by converting ethanol to acetic acid. Ethanol, acetic acid, and gluconic acid are the main products kombucha tea fermentation (Velićanski et al., 2014; Vazquez-Cabral et al., 2014).

Keywords: identification microorganisms; isolation molecular; acetic acid; phylogenetic identification; molecular phylogenetic; acid

Journal Title: Food Science and Technology International
Year Published: 2021

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