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A quantitative multiplex real-time PCR procedure was developed to monitor the dynamics in Norway spruce-Heterobasidion annosum pathosystem. The assay reliably detected down to 1 pg of H. annosum DNA and… Click to show full abstract
A quantitative multiplex real-time PCR procedure was developed to monitor the dynamics in Norway spruce-Heterobasidion annosum pathosystem. The assay reliably detected down to 1 pg of H. annosum DNA and 1 ng of host DNA in multiplex conditions. As a comparative method for quantifying fungal colonization, we applied the ergosterol assay. There was a very high correlation between the results obtained with the two methods, this strengthening the credibility of both assays. The advantages and disadvantages of these assays are discussed.
Journal Title: Plant protection science
Year Published: 2017
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