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Characterisation of a PR-1: Luciferase transgenic line deployed to uncover novel defence-related Arabidopsis mutants by luciferase imaging

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In order to identify components of the defence signalling network that may contribute to the establishment of disease resistance, we generated a novel PR-1::Luciferase transgenic line which was deployed in… Click to show full abstract

In order to identify components of the defence signalling network that may contribute to the establishment of disease resistance, we generated a novel PR-1::Luciferase transgenic line which was deployed in an imaging based screen to uncover novel defence-related mutants. Approximately, 5 000 ethylmethane sulfonate (EMS) lines and 30 000 activation tagged lines were generated and screened for enhanced LUC activity via ultra low light imaging.

Keywords: line deployed; defence; novel defence; transgenic line; uncover novel; luciferase transgenic

Journal Title: Plant protection science
Year Published: 2017

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