LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Molecular detection and phylogenetic analysis of avian reticuloendotheliosis virus from formalin fixed tissue by PCR in fancy chicken

Photo by heytowner from unsplash

The present study was carried out to identify avian neoplastic viruses in the formalin fixed tissues of fancy chicken. The tissue samples collected during necropsy were examined by histopathology. It… Click to show full abstract

The present study was carried out to identify avian neoplastic viruses in the formalin fixed tissues of fancy chicken. The tissue samples collected during necropsy were examined by histopathology. It showed lymphoid and reticular cell infiltration in kidney, liver and lungs. For identification and differentiation of avian neoplastic viruses, PCR was performed using primer sets specific for Mareks disease virus, avian leukosis complex and reticuloendotheliosis virus. It was found that tumors were REV originated. Further confirmation, purified PCR product was subjected to sequencing. It showed 99% homology with other REV isolates available in the NCBI database. The present communication describes infection of a fancy chicken with REV on the basis of histopathological findings as well as molecular methods.

Keywords: fancy chicken; tissue; formalin fixed; virus; reticuloendotheliosis virus

Journal Title: Indian Journal of Animal Research
Year Published: 2018

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.