LAUSR

The current study aims to assessment the estrogen concentration in both ovaries and serum in the fetus of the Nili Ravi buffalo. For this purpose, 73 female fetuses were collected from Sihala abattoir Islamabad, Pakistan. Both ovaries were obtained from fetuses with age ranging from day 51 to day 290 whereas; serum was available only for the fetuses ranging from the age of 146 days to 290 days. Radio immunoassay was used to measure the estrogen concentration and the data was analyzed through regression analysis of variance. Analysis of the data revealed mean ovarian estrogen concentration as 16.08±1.87 pg/mg of ovaries during the period of 51 to 290 days with peak value of 37.49±10.81pg/mg at 191-210 days. Ovarian estrogen concentration showed significant (P<0.05) increase with the advancing age of the fetus. On the other hand, mean serum estrogen concentration observed was 476.20 pg/ml during 146 to 290 days of fetal life with peak value of 734.11 pg/ml at the age group of 231-250 days. It can be concluded that both fetal ovarian and serum estrogen concentration increases with the advancing age of fetus in Nili-Ravi buffalo.