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ABSTRACT Zhang, S.;Wu, W.; Zheng, Q., and Wang, M., 2018. Effect of dispersants on bio-degradation of marine spilled oil. In: Liu, Z.L. and Mi, C. (eds.), Advances in Sustainable Port and Ocean Engineering. Journal of Coastal Research, Special Issue No. 83, pp. 364–368. Coconut Creek (Florida), ISSN 0749-0208. Light crude oil and heavy crude oil are selected to stimulate spilled oil pollution, mixed ratio of nonionic surfactant agent in dispersants of spilled oil is designed to determine the formula of efficient low temperature environment-friendly dispersant and the suitable application conditions, the effect of dispersant dosage, water salinity and temperature on the emulsification rate of spilled oil. Enriched oil degrading mixed bacteria is selected as the bio-degradation bacteria. Effect of dispersant dosage on bio-degradation of crude oil and influence of adding nutrient salts on bio-degradation of crude oil after emulsification of dispersants are studied by batch shaking experiment. Experimental results show that adding proportional dispersants is beneficial to the growth and propagation of microorganisms and the degradation of crude oil. The addition of nutrient salts can also significantly improve the bio-degradation rate of dispersants after emulsification of dispersants.