LAUSR

Biochar can facilitate the microbial reduction of various pollutants in soil and groundwater environments, but its impact on Cr(VI) reduction by dissimilatory metal reducing bacteria (DMRB) remains to be systematically investigated. In this study, we prepared biochars at 500 °C and 700 °C from wheat straw and grass, and investigated the impact of these biochars on Cr(VI) reduction by a model DMRB, Shewanella Putrefaciens CN32 (CN32). Pristine biochars abiotically reduced Cr(VI), which decreased the concentration and toxicity of chromium to CN32 cells, and brought about higher overall Cr(VI) removal extent after CN32 were added sequentially; on the other hand, no enhancement effect were observed when biochars and CN32 were added simultaneously. Further tests between biologically reduced biochars and Cr(VI) revealed that the reaction rates between bioreduced biochars and Cr(VI) are relatively sluggish compared to that of direct Cr(VI) reduction by CN32, which prohibited biochars from directly accelerating the Cr(VI) reduction by CN32 in simultaneous-addition scenario. The relative importance of biochars' surface functional groups and surface areas on their reactivities towards Cr(VI) reduction were also investigated. This study deepened our understanding towards the role of biochar played during bacterial Cr(VI) reduction and could potentially contribute to optimizing the biochar-based Cr(VI) bioremediation strategies.