LAUSR

Planar biaxial testing is a popular experimental technique for characterizing and comparing biological soft tissues. A correct identification of the different stress states of the tissue sample is therefore essential. However, the difference between the zero-stress reference state and the sample state prior to the loading cycle caused by the mounting, preconditioning and preloading is often not considered. The importance of this difference, caused by prestretch, is investigated by simulating virtual planar biaxial experiments, either assuming an ideal test with a single deformation gradient or using finite element modeling to simulate a rake-based experiment. Multiple parameter fitting methods are used to estimate the material properties based on the available experimental data. These methods vary based on how they approximate the zero-stress state: either the prestretch is ignored, or the loads are zeroed after the preload has been reached, or the unknown prestretch values are included into the optimization function. The results reveal the high necessity of assessing the stress-free state when analyzing a planar biaxial test. The material fitting including the prestretch outperforms the other methods in terms of correctly describing the mechanical behavior of the tested material. It can be extended to correct for the boundary effects induced by the gripping mechanisms, providing a more accurate, yet more computationally expensive estimate of the material properties.