LAUSR

Optimal conditions for resting-state functional magnetic resonance imaging (rs-fMRI) are still highly debated. Here, we comprehensively assessed the effects of various rest conditions on all cortical resting-state networks (RSNs) defined by an established atlas. Twenty-two healthy college students (22 ± 4 years old, 12 females) were scanned on a GE 3T MRI scanner. Rs-fMRI datasets were collected under four different conditions for each subject: (1) eyes open in dim light (Eyes-Open), (2) eyes closed and awake (Eyes-Closed), (3) eyes closed while remembering four numbers through the scan session (Eyes-Closed-Number) and (4) asked to watch a movie (Movie). We completed a thorough examination of the 17 functional RSNs defined by Yeo and colleagues. Importantly, the movie led to changes in global connectivity and should be avoided as a rest condition. Conversely, there were no significant connectivity differences between conditions within the frontoparietal control and limbic networks and the following subnetworks as defined by Yeo et al.: default-B, dorsal-attention-B and salience/ventral-attention-B. These were not even significant when compared to the highly stimulative Movie condition. A significant difference was not found between Eyes-Closed and Eyes-Closed-Number conditions in whole-brain, within-network and between-network comparisons. When considering other rest conditions, however, we observed connectivity changes in some subnetworks, including those of the default-mode-network. Overall, we found conditions with more external stimulation led to more changes in functional connectivity during rs-fMRI. In conclusion, the comprehensive results of our study can aid in choosing rest conditions for the study of overall and specific functional networks.