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Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes. Here, we… Click to show full abstract
Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes. Here, we demonstrate a simple and reliable method to determine the pH optimum for pyruvate kinase using the pyruvate kinase/lactate dehydrogenase coupled enzyme assay. This simple and reliable method can be broadly adapted to determine the pH optimum for various enzymes that are assayed using a coupled enzyme assay.
Keywords:
optimum coupled;
simple reliable;
coupled enzyme;
reliable method;
determination optimum
Journal Title: BioTechniques
Year Published: 2020
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Journal Title: BioTechniques
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!