LAUSR

BACKGROUND Bacteriocins (Bac1, Bac2, and Bac3) from Weissella confusa MBF8-1, weissellicin-MBF, have been reported as potential alternative substances as well as complements to the existing antibiotics against many antimicrobial-resistant pathogens. Previously, the genes encoded in the large plasmid, pWcMBF8-1, and the spermicidal activity of their synthetic peptides, have been studied in originally discovered Indonesia. Three synthetic bacteriocins peptides of this weissellicin-MBF have been reported as well for their potential activities, i.e. antibacterial and spermicidal. OBJECTIVE The aim of this study was to construct the recombinant Bacteriocin (r-Bac) genes, as well as to investigate the genes expressions and their functional analysis. METHOD Here, we constructed the recombinant Bacteriocin (r-Bac) genes and produced the recombinant peptides (r-Bac1, rBac2, and r-Bac3) in B. subtilis DB403 cells in a large scale. After purification using the His-tag affinity column, their potential bioactivities measured as antibacterial minimum inhibitory concentrations against Leuconostoc mesenteroides and Micrococcus luteus were determined. RESULTS Pure His-tag-recombinant Bac1, Bac2, and Bac3 were obtained and they could inhibit the growth of L. mesenteroides and M. luteus. CONCLUSION The recombinant bacteriocin could be obtained although with weak activity in inhibiting gram-positive bacterial growth.