LAUSR

Aim： As a new generation of blood substitutes, polyHb-SOD-CAT-CA has shown good application prospects in the existing research and explorations, resulting in the necessity of research on its process and preparation technology for future development. INTRODUCTION To explore the alternative to extractants for the preparation technology of polyHb-SOD-CAT-CA derived both from bovine blood and human cord blood, compared to the toluene currently used in the existing studies, several organic solvents, including n-hexane and ethyl ether, were investigated during the polyHb-SOC-CAT-CA preparation process. METHODS During the technological process, the effects of investigated organic extractants on the properties and stability of hemoglobin and enzymes, including SOD, CAT and CA, were investigated by monitoring the property indexes of macromolecules, including Hb concentrations, MetHb contents, molecular weight distribution, oxygen affinity of Hb, enzymes activities, and so on. RESULTS Among the investigated experimental groups, the results, including the Hb recoveries, MetHb contents, oxygen affinity, the molecular weight distribution of obtained complex, and enzyme activity, presented to be the best in n-hexane groups, followed by those in toluene groups, and these results in ether groups showed to be the worst. Meanwhile, during the preparation process, both for bovine and human cord derivatives, the changing of properties indexes of Hb and enzymes investigated demonstrated similar declining trends on the whole, and the oxygen-carrying properties and enzyme activities showed to be in the effective range. CONCLUSION Among the investigated organic extractants, both for preparation of bovine and human cord-derived polyHb-SOD-CAT-CA complex, n-hexane indicated to have much less negative influence on the properties and stability of Hb and enzyme molecules, including SOD, CAT, and CA. Moreover, the obtained human cord blood polyHb-SOD-CAT-CA also showed effective oxygen-carrying properties and enzyme activities, suggesting the promising application prospects of polyHb-SOD-CAT-CA and the new generation HBOC products in the future.