LAUSR

BACKGROUND Artabotrys odoratissimus (Annonaceae) is a medicinal and ornamental plant widely cultivated in Southeast Asia for its famous ylang ylang essential oil. The fruits of this plant are used for health benefits, but very little is studied about the bioactive principles, their role in regulating oxidative stress and tumour progression. OBJECTIVE The study aimed at evaluating the antiproliferative effects of fruit extract of Artabotrys odoratissimus and its bioactive fraction using cell-based assays. METHODS The free radical scavenging and anti-proliferative effects of Artabotrys odoratissimus Fruit Ethyl acetate (FEA) extract and its bioactive fraction were evaluated using Cell viability assays, Colony formation assay, Double staining assay, Reactive Oxygen Species (ROS) assay, Comet assay, Cell cycle analysis, and Western blotting. RESULTS The extract showed phenolic content of 149.8±0.11µg/mg Gallic acid equivalents and flavonoid content of 214.47±4.18 µg/mg Quercetin. FEA showed IC50 value of 76.35 µg/ml in ABTS assay and an IC50 value of 134.3±7.8 µg/ml on MIA PaCa-2 cells. The cells treated with 125 µg/ml and 250 µg/ml FEA showed increased apoptotic cells in Double staining assay, DNA damage during comet assay, attenuated ROS and cell cycle arrest at G2M phase at 125 µg/ml and 250 µg/ml. The active fraction AF5 showed a IC50 value of 67±1.26 µg/ml on MIA PaCa-2 cells during MTT assay, displayed potential antiproliferative effects, showed marked increase in the expression of γH2AX and p53. CONCLUSION These results prove that the fruit extract and the bioactive fraction demonstrate oxidative stress mediated DNA damage leading to the apoptosis in MIA PaCa-2 cell line.