LAUSR

AIM Aim of the present study is to develop and characterize capsaicin loaded Liposomes film (CAP-Lipofilm) for effective dermal delivery. METHODS The CAP-Liposomes were developed by ethanol injection technique and were characterized for particle size, polydispersity, zeta potential % entrapment efficiency and in vitro drug release studies by Franz diffusion cell. Based on optimum results (lowest particle size, highest zeta potential and highest % of entrapment efficiency and highest amount of drug release), the selected liposomes used for preparation of CAP-Lipo film. For comparative evaluation, The CAP-Lipo film and CAP-plain (capsaicin loaded film) were prepared solvent casting technique using PEO as the plain film base. RESULTS The liposomes particle size was found in the 234 to 380 nm, zetapotential was found in the range of -24.1 to -41.4 mV, %EE was found in the range of 40.67% to 76.52% respectively. The in vitro drug release CAP-liposomes shown in the range of 61% to 94% up to 12 hrs. The optimized F5 CAP-liposomes selected for Lipofilm. The plain, Lipofilm developed by film casting method and both films are evaluated against marketed patch (SALONPAS®). Based on results, CAP-liposomes and Lipofilm shown superior results compared to plain and marketed patch from in vitro/ ex vivo release. The drug and excipients compatibility by FTIR studies confirms the compatibility among the existed excipients and formulations. The stability studies confirms that 2-8°C condition is more favourable than accelerated condition respectively. CONCLUSION The CAP-Liposomes were successfully developed using ethanol injection method for transdermal delivery. It was found that CAP solubility was enhanced by intercalating in to the phospholipid fatty acids, increased encapsulation and loading efficiency. From the release studies, The Lipofilm shown prolonged permeation compared to marketed patch (SALONPAS®) and plain film across rat skin. Hence the liposomes are considered as an alternative potential tool to anti-inflammatory for effective dermal delivery.