LAUSR

Improving the genetic potential of animals using genomic technologies is an effective way to solve problems in domestic breeding of dairy breeds. The aim of the current study is to analyze the genetic diversity of Holstein cattle bred in the Tyumen Region using microsatellite markers. The blood samples of 241 Holstein cow were collected for genomic analysis. Genetic identification was performed using COrDIS kits. The resulting data indicated the presence of genetic diversity in the studied sample of dairy cattle bred at the Tyumen Region for a number of indicators. The number of alleles at 15 microsatellite loci in the tested breeds varied from 5 to 14. Eighteen unique allelic variants can be breed-specific markers for genetic identification of dairy cattle. The recorded data showed a high level of animal genetic variability. A high level of polymorphism (Ае 3.32) was detected in 47% of the loci under research. High values of the observed heterozygosity up to 0.91 at the Eth3 locus and of the polymorphism up to 0.77 at the Tgla53 locus were detected. In the results, 7% erroneous entries in the progeny pedigree was detected. The results of the current study contribute to the knowledge of genetic diversity of dairy cattle in the Tyumen region, Russia, and also paved the way to seek new relationships between allelic variants of microsatellite loci with the productive traits of animals.