LAUSR

Heart failure (HF) is the most common initial presentation of cardiovascular disease in type 2 diabetes (T2D) (1). Coronary microvascular dysfunction and compromised cardiac energy production have been proposed as pivotal features underpinning diabetic cardiomyopathy (2). Although functional alterations are highly prevalent in asymptomatic T2D patients, the relative associations of impaired cardiac energetics and perfusion to systolic and diastolic subclinical functional changes at rest and in response to acute hemodynamic stress in T2D have not been reported. Better understanding these relationships may lead to new therapeutic targets to prevent HF development in T2D patients. Using cardiovascular magnetic resonance (CMR) and P magnetic resonance spectroscopy (P-MRS), we assessed changes in cardiac energetics, perfusion, global longitudinal shortening (GLS), and systolic and diastolic function in response to increases in cardiac workload with dobutamine stress in T2D patients with overweight or obesity (n = 36) and nonathletic healthy volunteers (n = 20). Additionally, we compared results against those for 12 veteran athletes. The nonathletic healthy control group was selected because trained veteran endurance athletes are known to be markedly insulin sensitive. As a result, they represent an excellent control group for patients with T2D. Participants across the three groups showed similar age, sex, and ethnicity distribution. The BMI was significantly higher in the T2D group (with 10 being normal body weight, BMI 23 [22–24], and 26 being overweight, BMI 31 [29–32]). None of the participants had a documented history of cardiovascular disease (prior diagnosis of stroke, myocardial infarction, angina, moderate or severe valvular heart disease, atrial fibrillation, or any prior cardiovascular interventions), in line with the exclusion criteria. None of the participants reported exertional symptoms, and they were all considered class I based on New York Heart Association functional classification. Participants with T2D were free of diabetes complications as per exclusion criteria (retinopathy, nephropathy, or neuropathy) and were receiving only oral glucose-lowering treatments or diet control for the management of diabetes. Patients receiving insulin therapy were excluded from the study. Participants in the control groups were not receiving any medications. This prospective case–control study complied with the Declaration of Helsinki and was approved by the National Research Ethics Committee (approval no. 19/WM/0365). Informed written consent was obtained from each participant. The data will be shared on reasonable request to the corresponding author. For the stress protocol, intravenous dobutamine infusion up to 40 mg/kg/min was given to achieve a target heart rate of 65% of the age-predicted maximum. Mean rate pressure product (RPP) (RPP = systolic blood pressure × heart rate) was recorded at rest and under stress. Target heart rate was maintained for the duration of the P-MRS and dobutamine stress CMR acquisitions. The triglyceride index was calculated as a surrogate marker of insulin resistance, and plasma N-terminal prohormone B-type natriuretic peptide (NT-proBNP) concentrations were measured. Demographic, biochemical, and rest and stress CMR and P-MRS data are shown in Table 1. T2D patients showed significant reductions in resting energetics compared with the control groups, which confirmed the findings of previous studies. Increases in RPP with dobutamine stress were similar across study groups. In response to acute stress, further reductions in myocardial phosphocreatineto-ATP (PCr/ATP) ratios were seen in T2D patients but also to a similar relative extent in healthy volunteers and veteran athletes. The rest and stress left ventricular ejection fractions (LVEF) were similar across all groups, and all showed similar increments in LVEF with dobutamine stress. T2D patients showed significant reductions in GLS and mitral inflow E/A ratios at rest. During dobutamine stress, all groups showed similar increments in GLS and similar decrements in E/A ratio, but these parameters remained significantly higher in the two control groups. T2D patients showed lower stress