LAUSR

Syzygium cumini seed (L.) Skeels (myrtaceae) commonly known as “jamun” is widely used in Ayurveda. The main active constituents present in syzygium cumini seed is Ellagic and Ellagic acid. Syzygium cumini seed is official in Indian Ayurvedic pharmacopia. The comparative study of assay by HPTLC method and antibacterial activity was done for the Ellagic and Gallic acid and S.cumini extract at accelerated storage condition for a period of 6 months.The antibacterial activity of Ellagic acid, Gallic acid and ethanolic extract of dried seeds of s.cumini was determined by cup plate technique against gram-positive bacterial strain (Staphylococcus aureus) and gram-negative bacterial strain (Escherichia coli). For HPTLC method Ellagic acid, Gallic acid and extract was spotted on the plates precoated with silica gel 60 F254 and developed using toluene: ethyl acetate: formic acid, (6:6:1.5v/v/v) as mobile phase. Densitometry analysis was carried out at 271 nm. The method showed high sensitivity with good linearity over the concentration range of 200-1000ng/spot. The peak for Ellagic acid and Gallic acid were observed at Rf of 0.47 ±0.02 and 0.57±0.02 resp. The aim of our study was to observe the effect of accelerated storage on markers and extract. The analysis was carried out at 1,2,3,6 months study as per ICH guidelines for stability testing of drug at storage condition of 40oC ± 2oC/75% RH ± 5% RH. A decrease in antibacterial potential of the extract was observed with the simultaneous reduction in the % assay after a 6 month study. This method can be used for the quality control of the extract as well as markers.