LAUSR

Chitin obtained from the fermentation of Tiger Shrimp (Penaeus monodon) shell by using Providencia stuartii could be hydrolyzed into N-acetylglucosamine and another simple compound such as oligo-glucosamine. There is a limited research study regarding the isolation of oligo-glucosamine which also have a functional property. The study aimed to determine the best polar solvent and ratio sample and solvent (1:1 and 1:2, v/v) to precipitate oligo-glucosamine with the highest concentration. The polar solvents used in this study were ethanol, acetone, acetonitrile, ethanol-acetone (1:1, v/v), ethanolacetonitrile (1:1, v/v), and acetone-acetonitrile (1:1, v/v). Moreover, the yield obtained from precipitation by using those solvents were also investigated in this study. The result showed that combination of ethanol and acetonitrile (1:1, v/v) with ratio solvent-sample 1:1 (v/v) gave the highest concentration of oligo-glucosamine. The yield obtained was 0.12% (based on the volume obtained after fermentation) or 0.09% (based on the weight of chitin used for fermentation).