LAUSR

Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis and viral pneumonia in infants and young childrenworldwide. Currently no vaccine is available to prevent RSV infection, but virus-neutralizing monoclonal antibodies can begiven prophylactically, emphasizing the protective potential of antibodies. One concept of RSV vaccinology is mothers' immunization to induce high antibody titers, leading to passive transfer of high levels of maternal antibody to the fetus throughthe placenta and to the neonate through colostrum. Cotton rats are an excellent small animal model for RSV infection andhave been used to test maternal immunization. To mechanistically understand antibody transfer in the cotton rat model, wecharacterized the cotton rat placenta and Fc receptor localization. Placentas from cotton rats at midgestation (approximately day 14) and at late gestation (approximately day 25) and neonatal (younger than 1 wk) gastrointestinal tracts were collected for light microscopy, immunohistochemistry, and transmission electron microscopy. The cotton rat placenta is hemotrichorial and has 5 distinct layers: decidua, junctional zone, labyrinth, chorionic plate, and yolk sac. Consistent with the transfer of maternal antibodies, the majority of the Fc receptors are present in the yolk sac endoderm and fetal capillary endothelium of the chorionic plate, involving 10% of the cells within the labyrinth. In addition, Fc receptors are present on duodenal and jejunal enterocytes in cotton rats, similar to humans, mice, and rats. These findings provide the structural basis for the pre and postnatal transfer of maternal antibodies described in cotton rats.