LAUSR

This study was conducted to investigate the role of Na+ on ruminal short-chain fatty acid (SCFA) absorption and barrier function when isolated ruminal epithelium was exposed to high and low pH ex vivo. Nine Holstein steer calves (322 ± 50.9 kg of BW) consuming 7.05 ± 1.5 kg DM of a TMR were euthanized and ruminal tissue was collected from the caudal-dorsal blind sac. Tissues were mounted between 2 halves of Ussing chambers (3.14 cm2) and exposed to buffers that contained low (10 mM) or high (140 mM) Na+ with low (6.2) or high (7.4) mucosal pH. The same buffer solutions were used on the serosal side except that pH was maintained at 7.4. Buffers used to evaluate SCFA uptake contained bicarbonate to determine total uptake or excluded bicarbonate and included nitrate to determine non-inhibitable uptake. Bicarbonate-dependent uptake was calculated as the difference between the total and non-inhibitable uptake. Acetate (25 mM) and butyrate (25 mM) were spiked with 2-3H-acetate and 1-14C-butyrate, respectively, and were then added to the mucosal side, incubated for 1 min, and tissues were analyzed to evaluate rates of SCFA uptake. Tissue conductance (Gt) and the mucosal-to-serosal flux of 1-3H-mannitol were used to assess barrier function. There were no Na+ × pH interactions for butyrate or acetate uptake. Decreasing mucosal pH from 7.4 to 6.2 increased total acetate and butyrate uptake, and bicarbonate-dependent acetate uptake. Flux of 1-3H-mannitol was not affected by treatment. However, high Na+ concentration reduced Gt and prevented an increase in Gt from flux period 1 to flux period 2. The results of this study indicate that although providing more Na+ to the ruminal epithelium does not affect SCFA uptake or mannitol flux, it may help stabilize tissue integrity.