LAUSR

This study focuses on the effect of the modifier, pressure and temperature on the yield of extractable materials on the method of supercritical fluid CO2 extraction of the root of Far-Eastern wild Panax ginseng Meyer. The amounts of biologically active substances (ginsenosides) obtained with the use of small fractions of the modifier are very small, in contrast to large fractions of the modifier used in the experiment. Several experimental conditions were investigated: variations of pressure levels within the range of 200-400 bar, and ethanol percentages from 2 to 5% in the liquid phase at a temperature within the range of 31-70°C.During extraction process with variations in levels of pressure, temperature and amount of modifier, it was found that the presence of the modifier in the extracted system had a significant effect on the amount of extracted ginsenosides. The experimental procedures showed that the main factors that influence the result of ginseng extraction are the density of carbon dioxide, the diffusion coefficient of ginseng oil in carbon dioxide and the volatility of ginseng oil. A direct dependence of the yield of the extract on the amount of modifier was found. High-performance liquid chromatography of all extracts was performed and the quantitative content of ginsenosides in the obtained analytes was established.