LAUSR

The development of higher nutritional sorghum varieties requires the identification of high protein content germplasm that expands the genetic diversity of breeding programs. Therefore, a near-infrared (NIR) spectroscopy method was developed to predict the protein concentration of whole-grain sorghum with R2 = 0.83, root-mean-square error of prediction = 1.44%, and bias = 0.16%. We evaluated 228 tropical accessions from West and Central Africa maintained by the National Plant Germplasm System (NPGS) of the United States for protein content for three years. The analysis found that the protein concentrations among accessions varied from 5.05 to 15.00% with an average of 10.24%. Correlation analysis between years revealed changes in protein content and ranking across years for multiple accessions. However, heritability of protein concentration was moderately high (H2 = 0.81) indicating most of the observed variation could be determined by genetic differences among accessions. Sixteen tropical accessions with the highest protein concentration (>12.84%) may be used in breeding programs for the development of new and improved nutritional varieties. This assessment documented the range of natural variation for protein content in the NPGS sorghum tropical germplasm collection that could be used to enhance breeding programs focused on biofortification.