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In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel… Click to show full abstract
In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the native state of these structures. Green fluorescent protein showed functional state both on agarose gel and blotted membrane. Based on the combined procedures, we discovered conformation-specific monoclonal antibodies against PLXDC2 and SARS-CoV-2 spike protein.
Keywords:
conformation specific;
agarose native;
contact blotting;
gel;
gel electrophoresis;
native gel
Journal Title: Antibodies
Year Published: 2022
Link to full text (if available)
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Journal Title: Antibodies
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!