LAUSR

Enzyme-induced carbonate precipitation (EICP) is an emerging biogeotechnical technique that uses free urease to improve soil. Despite its advantages of eliminating complex microbial cultures and reducing reaction byproducts, its efficiency is considered lower than that of microbial induced calcite precipitation (MICP) due to the lack of nucleation sites that induce calcium carbonate deposition. To enhance the strengthening efficiency of EICP for fine-grained soils, an improved EICP method that involves adding an appropriate mass concentration of organic materials (skim milk powder, glutinous rice powder, and brown sugar) into urease solution was proposed and applied to reinforce silt in the Yellow River flood area of China. The preferred concentration and ratio of cementation solution and the optimum concentration of each of the organic materials were determined. Then, the reinforcement effect of the improved EICP at the optimum concentration was compared with the control group, and the reinforcement mechanism for this method was discussed. The results show that after the organic material inclusions, soil strength can be enhanced by 33% compared with EICP-treated soil and is nearly four times higher than that of untreated soil. The superiority of this method over traditional EICP and MICP mainly stems from its ability to provide templates and nucleation sites for calcium carbonate deposition and to improve the size, morphology, and structure of calcium carbonate crystals.