LAUSR

Fresh-cut vegetables are exposed to the risk of Salmonella spp. contamination. Effective sterilization methods and early warning systems play important roles in ensuring food safety of fresh-cut products. The aim of this study was to evaluate the effect of tea polyphenols (TP) combined with thermosonication (TS) treatment on inactivation of Salmonella enterica in fresh-cut wax gourd and to develop and estimate models using adaptive neuro-fuzzy inference system (ANFIS) with different membership functions (MFs) for predicting S. enterica population during storage at 25, 10, and 4 °C, respectively. The results showed that both TP and TS treatment can effectively reduce the population of S. enterica in fresh-cut wax gourd. The combination of TP (1.0%) and TS (50 °C, 1 min) treatment followed by storage at 4 °C may be a suitable bacteriostatic scheme for the preservation of fresh-cut wax gourd. Fluorescence microscopy analyses indicated that TP and TS treatment could lead to the destruction of the cell membrane, followed by the leakage of cytoplasm, and, finally, cell death. ANFIS with the gaussmf function performed well in modeling and predicting the population of Salmonella in fresh-cut wax gourd and provided a powerful tool for modelling and predicting microbe population and the shelf life of food products.