LAUSR

To reduce the considerable investments of toxicity testing and protecting animal welfare, a new toxicity testing strategy based on response pathways of human cell lines has been proposed in the United States to evaluate the chemical exposure risks to human health. However, the in vitro high-throughput assays have not yet been fully applied in ecotoxicity testing. This paper proposes a framework for high-efficiency ecotoxicity testing strategies to evaluate the ecological risk of chemicals. It consists of pathway-based toxicity testing, embryo-based toxicity testing, and predictive toxicology and data extrapolation, etc., according to different situations. The results of ecotoxicity testing or data mining are analyzed together with physicochemical properties, environmental fate, and exposure data of chemicals to conduct a comprehensive risk assessment of chemicals. The framework provides valuable points to establish high-efficiency ecotoxicity testing strategies in the 21st century.