LAUSR

Simple Summary Very little is known about how biostimulants such as amino acids affect the enzymatic activity of peppers. The three crucial enzymes for the synthesis and degradation of capsaicinoids were studied in a 72 h time course. The activity of the two crucial enzymes for the synthesis of capsaicinoids increased in the first hour after foliar application and then gradually decreased to the baseline activity. Placenta exhibited higher enzyme activity and capsaicinoid content. Since capsaicinoids are widely consumed by humans, the use of various natural substances called biostimulants could improve the overall quality of peppers, which would mean high-yield and high-quality fruits. Abstract Biostimulants are widely used in agriculture because they can improve fruit quality and quantity. Less is known about how biostimulants act over time in plants, in our case peppers, and how they affect the enzyme activity of important enzymes for capsaicinoid synthesis. The biostimulatory effects of amino acids on the activities of phenylalanine ammonia lyase (PAL), capsaicin synthase (CS), and peroxidase (POX) were investigated in the pericarp and placenta of the chili pepper Capsicum baccatum L. cv. “Bishop Crown” over 72 h of application. The PAL and CS activities significantly increased in the placenta after 1 h of biostimulant application, with significant increases of 130% and 16%, respectively. The POX activity remained unchanged over the full 72 h in the placenta but significantly increased after 48 h in the pericarp (+53%). Total capsaicinoids increased in the first hour of biostimulant application, by 4.30 g/kg FW in the placenta (19%) and by 0.94 g/kg FW in the pericarp (+56%). Biostimulant application also increased total and individual capsaicinoids after 48 h in the chili placenta and pericarp. With improved methods for enzymatic determination, we gained new insights into the responses of chilies to biostimulant amino acids.