LAUSR

Glucose oxidase (GOD) is widely used as an important oxidoreductase in various fields. However, maintaining the vitality and stability of GOD under environmental stress is a challenge. To improve the thermal and storage stability of GOD, this study constructed sodium alginate–carboxymethyl cellulose sodium gel particles (SA/CMC) and chitosan-coated SA/CMC gel particles (CS/SA/CMC) of GOD. The encapsulation efficiency (EE), gel particle structure, stability, and release behavior of GOD were evaluated. The results showed that the thermal stability of GOD encapsulated in SA/CMC and CS/SA/CMC gel particles was improved by approximately 2.8-fold and 4.3-fold compared with the free enzyme at 85 °C, respectively. In addition, CS/SA/CMC gel particles enhanced the enzyme activity retention rate of GOD to over 80% during storage at 4 °C for four weeks. Both SA/CMC and CS/SA/CMC gel particles loaded with GOD had more than 70% of the enzymes released during the simulated gastrointestinal experiment. The results demonstrated that encapsulating GOD in SA/CMC and CS/SA/CMC gel particles could improve its thermal stability and storage stability, which is conducive to further expanding the application of GOD in food, pharmaceutical and feed industries.