LAUSR

Simple Summary The juvenile hormones (JHs) play critical roles during insect development and reproduction. The numerous effects of JHs have generated multiple basic scientific questions, as well as prospects for the development of insecticidal endocrine disruptors. There is an increasing need for methods to identify and quantify endogenous JHs. The low titers and difficulties in working with these lipophilic compounds have often hindered the study of JH biology. In this article, we review the existing information on the detection and quantification of JH from insect samples, the development of approaches to manipulate JH titers, and the use of next-generation tools to modulate JH homeostasis. Abstract The juvenile hormones (JHs) are a group of sesquiterpenoids synthesized by the corpora allata. They play critical roles during insect development and reproduction. To study processes that are controlled by JH, researchers need methods to identify and quantify endogenous JHs and tools that can be used to increase or decrease JH titers in vitro and in vivo. The lipophilic nature of JHs, coupled with the low endogenous titers, make handling and quantification challenging. JH titers in insects can easily be increased by the topical application of JH analogs, such as methoprene. On the other hand, experimentally reducing JH titers has been more difficult. New approaches to modulate JH homeostasis have been established based on advances in RNA interference and CRISPR/Cas9-based genome editing. This review will summarize current advances in: (1) the detection and quantification of JHs from insect samples; (2) approaches to manipulating JH titers; and (3) next-generation tools to modulate JH homeostasis.