LAUSR

Simple Summary Among the investigative questions to define the temporal frame of a criminal event, the time since death plays a fundamental role. After death, the body goes through a series of physical and chemical transformations—known as decomposition. The way in which different parts of the body undergo these transformations can be quantified with a scale of scores (TBS, the total body score) and used for the time since death evaluation using the accumulated degree-days (ADDs) parameter, which accounts for time and temperature. This method is reported as TBS/ADD. Another way to estimate the time since death is based on the insect development on the body. Flies represent the first body coloniser and the development of their immature stages is used to define the time of colonisation that is temperature dependent and species specific. In this study, the two methods were compared based on 30 forensic cases occurring in northern Italy. The results highlighted the limits of the TBS/ADD method and the importance of the entomological approach, keeping in mind that with insects the colonization time is evaluated. This time is the minimum time since death. Abstract Establishing the post mortem interval (PMI) is a key component of every medicolegal death investigation. Several methods based on different approaches have been suggested to perform this estimation. Among them, two methods based their evaluation on the effect of the temperature and time on the considered parameters: total body score (TBS)/accumulated degree-days (ADDs) and insect development. In this work, the two methods were compared using the results of minPMI and PMI estimates of 30 forensic cases occurring in northern Italy. Species in the family Calliphoridae (Lucilia sericata, Calliphora vomitoria and Chrysomya albiceps) were considered in the analyses. The results highlighted the limits of the TBS/ADD method and the importance of the entomological approach, keeping in mind that the minPMI is evaluated. Due to the fact that the majority of the cases occurred in indoor conditions, further research must also be conducted on the different taxa to verify the possibility of increasing the accuracy of the minPIM estimation based on the entomological approach.