LAUSR

Simple Summary We present a morphological and morphometric study with T. garciabesi, T. guasayana, T. patagonica, and T. sordida sensu stricto species within the Triatoma genus. This group of species is important for the eco-epidemic scenario of Chagas disease in the Americas; their species have morphological, biological, and behavioral similarities that make diagnosis difficult. For the first time, the description of the female external genitalia by scanning electron microscopy (SEM), a character that has helped with the delimitation of species and genera in Triatominae, is published, in addition to presenting an extensive study with eggs, covering morphology and morphometry. The study with eggs is an important tool in taxonomic studies of the subfamily. In addition to taxonomic contributions, it was possible through the descriptions to corroborate the validity of T. garciabesi and confirm the current classification of these species. Abstract Triatoma is the most diversified and one of the most important genera from an epidemiological perspective. Given the difficulty in identifying some species of the Triatoma genus, morphological, histological, and morphometric studies were performed to provide new characters that make it possible to differentiate T. garciabesi, T. guasayana, T. patagonica, and T. sordida sensu stricto, triatomines that overlap geographically and have vector potential. Through the external female genitalia, as well as morphology, morphometry, and histology of eggshells, it was possible to discriminate the four species. In addition, this study reinforces the taxonomic validity of T. garciabesi and provides new data for discussion on systematic issues of T. guasayana and T. patagonica.