LAUSR

Simple Summary The mitochondrial genome (mitogenome) has been used extensively to investigate phylogenetic relationships at various taxonomic levels. Insect mitochondrial genomes are important for understanding insect evolution and relationships. We performed complete mitogenome sequencing and annotation for nine Meconematinae species and combined the data with data from previously assembled mitochondrial genomes in the family Tettigoniidae to conduct a phylogenetic analysis. The monophyly of Xizicus, Xiphidiopsis, and Phlugiolopsis was not well supported in our findings. Abstract Currently, the subfamily Meconematinae encompasses 1029 species, but whole-mitochondrial-genome assemblies have only been made available for 13. In this study, the whole mitochondrial genomes (mitogenomes) of nine additional species in the subfamily Meconematinae were sequenced. The size ranged from 15,627 bp to 17,461 bp, indicating double-stranded circular structures. The length of the control region was the main cause of the difference in mitochondrial genome length among the nine species. All the mitogenomes including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs) and a control region (CR). The majority strand encoded 23 genes, and the minority strand encoded 14 genes. A phylogenetic analysis reaffirmed the monophyletic status of each subfamily, but the monophysitism of Xizicus, Xiphidiopsis and Phlugiolopsis was not supported.