LAUSR

Simple Summary Corydalinae Davis, 1903, is a subfamily of Corydalidae in the order Megaloptera with 177 dobsonfly species. Larvae of Corydalinae are predatory and inhabit lotic habitats in montane areas. Mitochondrial genes might be exposed to differential selective pressures across populations or species exhibiting divergent biogeographical distributions or occupying distinct ecological niches. Comparative analyses of mitochondrial genomes were conducted using all published mitochondrial genomes of Corydalinae in this study. Non-synonymous to synonymous substitution rate ratio (Ka/Ks) analysis suggested that total protein-coding genes (PCGs) across species from eight genera of Corydalinae might be under purifying selection. Positive selection pressure analysis revealed potential positive selection sites in mitochondrial genes of five genera distributed in areas with significant annual temperature difference, which may be attributed to differential adaptive evolution in response to climatic factors.