LAUSR

Woodpeckers exhibit selectivity when choosing tree cavities for nest development in forest ecosystems, and fungi play a significant and important role in this ecological process. Therefore, there is a complex and intricate relationship between the various behaviors of woodpeckers and the occurrence of fungal species. Research into the complex bond between fungi and woodpeckers was undertaken to provide more information about this remarkable ecological relationship. Through the process of line transect sampling, woodpecker traces were searched for, and mist nets were set up to capture them. A total of 21 woodpeckers belonging to four species were captured. High-throughput sequencing of the ITS region was performed on fungal-conserved samples to enable an in-depth analysis of the fungal communities linked to the woodpeckers’ nests. Members of Ascomycota were the most abundant in the samples, accounting for 91.96% of the total, demonstrating the importance of this group in the forest ecosystem of this station. The statistical results indicate significant differences in the fungal diversity carried by woodpeckers among the different groups. Species of Cladosporium were found to be the most prevalent of all the detected fungal genera, accounting for 49.3%. The top 15 most abundant genera were Cladosporium, Trichoderma, Beauveria, Epicococcum, Hypoxylon, Penicillium, Nigrospora, Aspergillus, Oidiodendron, Cercospora, Talaromyces, Phialemo-nium, Petriella, Cordyceps, and Sistotrema. The standard Bray–Curtis statistical technique was used in a hierarchical clustering analysis to compute inter-sample distances, allowing for the identification of patterns and correlations within the dataset. We discovered that in the grouped samples from woodpeckers, there were differences in the diversity of fungal communities carried by four woodpecker species, but the less dominant fungal species were still similar. The findings highlight the need to consider these diverse ecological linkages in woodpecker research and conservation efforts.