Photo by marya_volk from unsplash
Graphene oxide (GO) is widely used in sensors. The detection of proteins based on bare GO has been developed; however, the detection sensitivity needs to be improved. In this paper,… Click to show full abstract
Graphene oxide (GO) is widely used in sensors. The detection of proteins based on bare GO has been developed; however, the detection sensitivity needs to be improved. In this paper, a novel GO-DNA sensor for thrombin detection was developed using an aptamer linked to the surface of GO. Polyethylene glycol (PEG) was further used to prevent thrombin from nonspecific adsorption and to improve the sensitivity of the sensor for detection of thrombin. In order to improve the limit of detection for thrombin, we developed a GO and RecJf exonuclease co-assisted signal amplification strategy, and a detection limit of 24.35 fM for thrombin was achieved using this strategy. The results show that it is a promising method in analytical applications.
Journal Title: Micromachines
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!