LAUSR

This research aimed to develop an FTIR-based method for rapid and low-cost integrated quality assessment of organic-inorganic composite herbs, which are kinds of herbs composed of both organic and inorganic active ingredients or matrix components. A two-step quality assessment route was designed and verified using the example of Indigo Naturalis (Qing Dai). First, the FTIR spectra were used as global chemical fingerprints to identify the true and fake samples. Next, the contents of the organic and inorganic marker components were estimated by FTIR quantification models to assess the quality of the true samples. Using the above approaches, all the 56 true samples and five fake samples of Indigo Naturalis could be identified correctly by the correlation threshold of the FTIR chemical fingerprints. Furthermore, the FTIR calibration models provided an accurate estimation of the contents of marker components with respect to HPLC and inductively coupled plasma optical emission spectrometry (ICP-OES). The coefficients of determination (R2) for the independent validation of indigo, indirubin, and calcium were 0.977, 0.983, and 0.971, respectively. Meanwhile, the mean relative errors (MRE) for the independent validation of indigo, indirubin, and calcium were 2.2%, 2.4%, and 1.8%, respectively. In conclusion, this research shows the potential of FTIR spectroscopy for the rapid and integrated quality assessment of organic-inorganic composite herbs in both chemical fingerprints identification and marker components quantification.