LAUSR

Although surface-enhanced Raman scattering (SERS) technology has been widely explored nowadays in various fields, the fabrication of practical SERS-active substrates with prominent recognition ability for various analyte molecules is still defective. Natural Fulfora candelaria wing (FCW) with three-dimensional (3D) hierarchical reticular nanostructure was selected as a new bioscaffold for rough silver (Ag) nanoislands to be assembled on to prepare a practical SERS substrate (Ag/FCW substrate). By adjusting the sputtering time of metal Ag, the morphology of the substrates could be easily tuned to control the formation and distribution of “hot spots”. Three-dimensional finite-difference time-domain (3D-FDTD) simulation indicated that the excellent SERS performance under optimal morphology was ascribed to the local enhanced electric field in rough Ag surface and effective “hot spot” areas. The SERS measurement results show that the optimal Ag/FCW substrates had high SERS performance in terms of Raman signal sensitivity, reproducibility, uniformity and recognition ability for various analyte molecules. Coupled with flexibility of the biological substrates and the cost effectiveness, the sensitive SERS detection of varied analytes based on Ag/FCW substrates offered great potential for practical applications.