LAUSR

Zearalenone (ZEN) is widely found in food and feed. Its cytotoxicity, reproductive toxicity, genetic toxicity, immunotoxicity and hepatorenal toxicity have serious impacts on human and animal health. In order to help animals avoid ZEN poisoning in feed, ZEN-degrading bacterial strains were screened from fecal samples through a zearalenone challenge pig model, and their degradation characteristics were researched. Through the optimization of parameters such as the culture time, pH value, temperature, and strain concentration, the optimal conditions for the ZEN-degrading ability of these strains were preliminarily determined, and the active site of the ZEN degradation was explored. In this study, three strains (SY-3, SY-14, SY-20) with high ZEN degradation capacities were obtained. SY-3 was identified as Proteus mirabilis, and its main degrading component was the supernatant. SY-14 and SY-20 were identified as Bacillus subtilis. Their main degrading components were the intracellular fluid of SY-14, and the intracellular fluid and cell wall of SY-20. The above results showed that the ZEN challenge model was an effective way to screen ZEN-degrading bacteria.