LAUSR

Simple Summary Identification of markers to include in breeding plans is necessary in order to develop disease resistance to infectious diseases utilizing genetic control approaches. Recent studies used genome-wide association analysis to find new genes primarily responsible for the mastitis susceptibility of dairy cattle. Results, however, did not entirely persuade us that these genes were good candidates because we were unable to corroborate previously discovered SNP variants or genomic areas. In this study, SNPs linked to mastitis resistance/susceptibility were discovered in the RASGRP1, NFkB, CHL1, MARCH3, PDGFD, MAST3, EPS15L1, C1QTNF3, CD46, COX18, NEURL1, PPIE, and PTX3 genes by PCR-DNA sequencing in Holstein and Montbéliarde cows with and without mastitis. The mRNA levels of these indicators also varied between healthy and affected dairy cows. Therefore, it may be useful to discover potential genes linked to mastitis susceptibility to improve the effectiveness of animal selection for innate resistance. Abstract A major factor in the propagation of an infectious disease is host genetics. In this study, 180 dairy cows (90 of each breed: Holstein and Montbéliarde) were used. Each breed’s tested dairy cows were divided into two groups of comparable size (45 cows each), mastitis-free and mastitis-affected groups. Each cow’s jugular vein was punctured to obtain blood samples for DNA and RNA extraction. In the examined Holstein and Montbéliarde dairy cows, single nucleotide polymorphisms (SNPs) related with mastitis resistance/susceptibility were found in the RASGRP1, NFkB, CHL1, MARCH3, PDGFD, MAST3, EPS15L1, C1QTNF3, CD46, COX18, NEURL1, PPIE, and PTX3 genes. Chi-square analysis of identified SNPs revealed a significant difference in gene frequency between mastitic and healthy cows. Except for CHL1, mastitic dairy cows of two breeds had considerably higher mRNA levels of the examined genes than did healthy ones. Marker-assisted selection and monitoring of dairy cows’ susceptibility to mastitis may be accomplished through the use of discovered SNPs and changes in the gene expression profile of the studied genes. These findings also point to a possible method for reducing mastitis in dairy cows through selective breeding of animals using genetic markers linked to an animal’s ability to resist infection.