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Evaluation of a Point-of-Care Feline Immunodeficiency Virus (FIV) Test Kit (RapidSTATUS™ FIV) to Determine the FIV Status of FIV-Vaccinated and FIV-Unvaccinated Pet Cats in Australia

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Simple Summary This study evaluated a commercial point-of-care (PoC) feline immunodeficiency virus (FIV) test kit (RapidSTATUS™ FIV) for its accuracy in determining the FIV status of FIV-vaccinated and FIV-unvaccinated pet… Click to show full abstract

Simple Summary This study evaluated a commercial point-of-care (PoC) feline immunodeficiency virus (FIV) test kit (RapidSTATUS™ FIV) for its accuracy in determining the FIV status of FIV-vaccinated and FIV-unvaccinated pet cats in Australia. In countries where FIV vaccination is used, veterinarians need a PoC kit that will produce a negative result for a FIV-uninfected cat, even if the cat is FIV-vaccinated or the FIV vaccination history is unknown, since incorrect diagnoses can impact negatively on the welfare of cats. FIV PoC kits also need to produce positive results in FIV-infected cats to help with appropriate management and to enable strategies to be implemented to prevent other cats from becoming FIV-infected. Results presented here show RapidSTATUS™ FIV to be highly accurate (98.8−100%) in a range of FIV-vaccinated and FIV-unvaccinated scenarios. Therefore, Australian veterinarians can reliably use RapidSTATUS™ FIV to rapidly and accurately determine the FIV status of all cats. Abstract Feline immunodeficiency virus (FIV) is a retrovirus that can cause immunosuppression, co-morbidities, and neoplasia in infected cats, and is commonly tested for in veterinary clinics and animal shelters in Australia. FIV diagnosis using point-of-care (PoC) kits to detect FIV antibodies in Australia is complicated by the commercial availability of an inactivated whole-FIV vaccine. The aim of this study was to determine the accuracy of the RapidSTATUS™ FIV antibody test kit in FIV-vaccinated and FIV-unvaccinated cats in Australia. Plasma from pet cats of known FIV vaccination and FIV infection statuses (n = 361), comprised of 57 FIV-uninfected cats annually vaccinated against FIV, 10 FIV-uninfected cats with lapsed FIV vaccination histories, 259 FIV-unvaccinated/FIV-uninfected cats, and 35 FIV-infected cats, was tested. RapidSTATUS™ FIV testing had sensitivity of 97.1% (34/35) and specificity of 100% (326/326), with an overall accuracy of 99.7% (360/361). Additional testing was undertaken using plasma from FIV-uninfected cats recently administered a primary FIV vaccination course (n = 12) or an annual booster FIV vaccination (n = 10). RapidSTATUS™ FIV was 98.8% (81/82) accurate and 100% (32/32) accurate in cats recently administered primary or annual FIV vaccinations, respectively. The high level of accuracy of RapidSTATUS™ FIV (98.8–100%) therefore establishes this PoC kit as a DIVA (differentiating infected from vaccinated animals) test. RapidSTATUS™ FIV is recommended to aid animal shelters, veterinarians, and researchers in Australia to accurately determine FIV infection status, irrespective of FIV vaccination history.

Keywords: fiv unvaccinated; fiv vaccinated; rapidstatus fiv; fiv; vaccinated fiv

Journal Title: Veterinary Sciences
Year Published: 2022

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