LAUSR

Chemicals and reagents Liensinine (CAS: 2586-96-1) was purchased from CHENGDU MUST BIO-TECHNOLOGY CO., LTD (Chengdu, China), purity >98%, molecular formula: C37H42N2O6, molecular weight: 610.3. LPS (Escherichia coli O111: B4) was purchased from Sigma-Aldrich (St Louis, USA). Primary antibodies against COX-2 (12375-1-AP), GAPDH (60004-1-Ig), Nrf2 (16396-1-AP), Bax (50599-2-Ig), and P62 (18420-1-AP) were purchased from Proteintech (Wuhan, China). Primary antibodies against Keap 1 (WL03285), Bcl-2 (WL01556), and Caspase 3/cleaved Caspase 3 (WL02117) were provided by Wanleibio (Shenyang, China). Primary antibody against p-NF-κB p65 (3033) was purchased from Cell Signaling (Beverly, USA). Primary antibody against LC3 B (T55992) was purchased from Abmart (Shanghai, China). Anti-mouse (SA00001-1) and anti-rabbit (SA00001-2) horseradish peroxidase-conjugated secondary antibodies were purchased from Proteintech.