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The Black Sea offers numerous harnessing possibilities for the medical and pharmaceutical, agricultural, food industry and cosmetic fields. Collagen extraction from the Black Sea fish is a research area of… Click to show full abstract
The Black Sea offers numerous harnessing possibilities for the medical and pharmaceutical, agricultural, food industry and cosmetic fields. Collagen extraction from the Black Sea fish is a research area of great interest. The purpose of this paper is to optimize the collagen quantitative analysis method based on hydroxyproline reagent through visible molecular absorption spectrometry. The adapted method was validated, achieving the following performance criteria: linearity, detection and quantification limits, accuracy/fidelity, stability/sturdiness, repeatability, and measurement uncertainty. The validated method was applied for the quantitative determination of collagen content in Grey Mullet fish and for the evaluation of collagen extraction output.
Journal Title: Revista de Chimie
Year Published: 2019
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