LAUSR

Objectives:(Investigation the cytotoxicity of total alkaloid extract of Peganum harmala L. seeds against tumor cell lines. Methods: From the seeds of P. harmala. total alkaloid was extracted using 80%) methan0l, chloro forme(at pH2 and pH10 and they chloro forme partn was driedd to gettinge the extract ofy totaly alkaloidd t. The totaly alkaloidss werey revealing ofd 1qualitatively by Dragend0rff’s, Mayer.’s and Hage.r‘s) 1reagents and estimated 1quantitatively by Bromo cresol green spectro.photometry depending on the curve of atropine 1calibration. (The activity of cytotoxicity was achieved by using Michigan Cancer Foundation-7 (MCF-7) breast cancer cell line and fetal hepatic cell line (WRL-68) non-tumorigenic by MTT assayi. Resultse: Thet t0tal content of alkal0id of P. harmala extract was 328.62± 2.8mg/100 g dry weight of plant. This extract drop the viability of cells in b0th cell lines, the greatest reduction happened in the concentration 400 μg/ml was 60.2± 2.8 % for MCF-7 and 66.5±2.2% for WRL-68. Conclusion: (The alkaloids of P. harmala had variable effects againstt cancery andy normale cellt linesy 1depending 0n thei type of alkal0id compounds andi their concentration in the extract).