LAUSR

Background: Rumex vesicarius L. (Polygonaceae), an edible plant, is documented to have many bioactivephytochemicals. In spite of it fully -known antioxidant efficacy of crude extract Rumex vesicarius but stillhas the cytotoxicity.Methodology: Methanolic extraction was prepared from leaves of Rumex vesicarius and was checked byGC/MS. Two parts of assay were classified as : First, evaluation antioxidant potency using DPPH freeradicals, while the two assay of MTT was utilized to determine anti-cancer effect on two different cell lines(MCF-7 and WRL68) for different concentrations (400?g/ml, 200?g/ml, 100?g/ml, 50?g/ml and 25?g/ml and 6.2 ?g/mL) and identified the efficacy of the crude extract on MCF-7 cell morphology, response tostress, potential of mitochondria and viability were evaluated with High Content Scanning and MTT.Results: GC/MS analysis showed the methanol extract of Rumex Vesicarius L. had the highest percentageof L- Pidolic Acid (35.98%), vitamin E (12.05%). R. Vesicarius showed antioxidant activity which increasedwhen increasing of concentration, R. Vesicarius effect ranged from (75.935,9.96%). The results showedRumex vesicarius ability to inhibit cellular growth of both cell lines (WRL68 and MCF-7). This inhibitionincreases with increasing concentration. The best inhibition of growth was at 400?g/ml of Methanol extract.The MCF-7 cell line was more effective than the WRL68 cell line, High Content Screening (HCS) assay of200?g/ml, 100?g/ml, 50?g/ml, 25?g/ml and 12.5?g/ml of methanol extract of R. Vesicarius showed toxiceffects toward MCF-7 cell line after 24 hours of treatment in a dose-dependent manner with a reduction inthe number of total cell count, reduction in the mitochondrial membrane potential (MMP), an increase in thenuclear intensity, increase in the membrane potential and increase in cytochrome C.Conclusion: Methanolic extract of Rumex vesicarius contains many bioactive phytochemicals and appearto have cytotoxic and antioxidant activity against MCF-7 & WRL68 Cell line.