LAUSR

Objective To investigate the function characteristics of CD4 T cell converted double negative T cell and provide a basis for further insight into the characteristics of mouse converted double negative T cell. Methods The gene expression profile was analyzed by transcriptome sequencing and protein mass spectrometry. The expression of cell active marker CD44, CD69 and OX40 was investigated by flow cytometry and the cytotoxicity of mouse double negative T cell was verified by CFSE staining. Results Mouse CD4 T cell converted double negative T cell expressed cell phenotype that differed from other mature CD4 T cells. Mouse converted double negative T cell expressed high level of active marker of CD44, CD69 and OX40. Cytotoxicity of PrfKO DN T was significantly reduced. Conclusions Mouse CD4 T cell converted double negative T cell has distinguishing cell phenotypes, that are not identical to other mature CD4 T cells. Mouse double negative T cell overexpresses cell activation marker and cytotoxic cytokines. The immune suppressive function of mouse double negative T cell is mainly dependent on perforin pathway. Key words: T-Lymphocytes; Double negative T cell; Cell transformation, neoplastic; Transcriptome sequencing